Chain of the intermediate CA support in Istio

I am trying to get the information regarding number of Intermediate CAs can be configured as Chain of the intermediate CA support in Istio? I am trying to setup PKI as below:

Root CA
Intermediate CA 3
Intermediate CA 2
Intermediate CA 1
Cluster 1 CA, Cluster 2 CA … Cluster N CA

Is it supported by istio? If yes, then Is there any limit to support number of Intermediate CAs by Istio?